廖鲁剑 博士 功能蛋白质组学

脑功能基因组学教育部重点实验室PI
生命科学学院 教授
办公电话：021-24207044
Email: ljliao@bio.ecnu.edu.cn

研究方向
以功能性蛋白组学为主要研究手段，进行神经系统疾病的蛋白质表达异常及其信号通路研究。基于大分子质谱学方法能对及其复杂的生物样品进行大规模的蛋白质表达分析，实现蛋白质的翻译后修饰检测。同时应用大分子质谱学技术研究生物大分子的相互作用及在其他系统的蛋白质差异表达。这些重要的分析手段应用到重大疾病能发掘潜在的疾病发生发展的机制，亦能为临床早期诊断提供依据。

个人简历
1988.9-1993.7          学士         湖南医科大学，医学检验
1995.9-1998.7          硕士         华西医科大学，生物化学
1998.8-2002.12        博士         鲍林格林州立大学，生命科学，美国俄亥俄州
2003.2 - 2004.12    博士后  美国艾默蕾大学
2005.1 - 2007.3        博士后  美国斯特里普斯研究所
2007.4 - 2012.7        Staff Scientist 美国斯特里普斯研究所
2012.8 - 2013.7        中科院上海生命科学院生物化学与细胞生物学研究所
2013.8 – 至今         华东师范大学生命科学院

学术任职/人才获奖
上海市“浦江人才”

代表性成果
Note: * Co-first author, # corresponding author.

1. Tang B, Wang T, Wan H, Han L, Qin X, Zhang Y, Wang J, Yu C, Berton F, Francesconi W, Yates JR 3rd, Vanderklish PW, and Liao L#. Fmr1 deficiency promotes age-dependent alterations in the cortical synaptic proteome. Proc Natl Acad Sci U S A. 2015; doi:10.1073/pnas.1502258112
2. Qin X, Zheng C, Yates JR 3rd, Liao L#. Quantitative phosphoproteomic profiling of PINK1-deficient cells identifies phosphorylation changes in nuclear proteins. Mol Biosyst. 2014 10(7):1719-29.
3. Li K, Zhou T, Liao L, Yang Z, Wong C, Henn F, Malinow R, Yates JR, Hu H. βCaMKII in Lateral Habenula Mediates Core Symptoms of Depression.  Science 2013;341:1016-1020.
4. Sando R 3rd, Gounko N, Pieraut S, Liao L, Yates JR 3rd, and Maximov A. HDAC4 Governs Transcriptional Programs Essential for Synaptic Development and Function. Cell. 2012；151(4):821-34.
5. Banerjee S, Liao L, Russo R, Nakamura T, McKercher SR, Okamoto S, Haun F, Nikzad R, Zaidi R, Holland E, Eroshkin A, Yates JR 3rd, Lipton SA. Isobaric tagging-based quantification by mass spectrometry of differentially regulated proteins in synaptosomes of HIV/gp120 transgenic mice: Implications for HIV-associated neurodegeneration. Exp Neurol. 2012; 236(2):298-306
6. McClatchy DB, Liao L, Lee JH, Park SK, Yates JR 3rd. Dynamics of subcellular proteomes during brain development. J Proteome Res. 2012; 11(4):2467-79
7. Liao L, Sando RC, Farnum JB, Vanderklish PW, Maximov A, and Yates JR 3rd. 15N labeled brain enable quantification of proteome and phosphoproteome in neurons. J Proteome Res. 2012; 11(2):1341-53
8. Mcclatchy D*, Liao L*, Park SK, Xu T, Lu B, and Yates JR. Differential proteomic analysis of mammalian tissues using SILAM. Plos One. 2011; 6(1): e16039
9. Liao L*, McClatchy DB*, Yates JR. Primer: shotgun proteomics in neuroscience. (Review) Neuron. 2009; 63(1):12-26
10. Park SK*, Liao L*, Kim JY, and Yates JR. A computational approach to correct arginine-to-proline conversion in quantitative proteomics. Nature Methods. 2009; 6(3):184-185
11. Liao L, Park SK, Xu T, Vanderklish PW, and Yates JR. Quantitative proteomic analysis of primary neurons reveals diverse changes in synaptic protein content in fmr1 knockout mice. Proc Natl Acad Sci USA. 2008; 105(40):15281-6
12. Liao L*, McClatchy DB*, Park SK, Xu T, Lu B and Yates JR. Quantitative analysis of brain nuclear phosphoproteins identifies developmentally regulated phosphorylation events. J Proteome Res. 2008; 7(11):4743-55
13. Xia Q*, Liao L*, Cheng D, Duong D, Gearing M, Lah JJ, Levey AI, and Peng J. Identification of novel kinases and ubiquitin ligases associated with Lewy bodies by comparative proteomics. Frontiers in Bioscience. 2008; 13:3850-6
14. Choe E, Liao L, Zhou J, Cheng D, Duong D, Jin P, Tsai L, and Peng J. Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1. J Neurosci. 2007; 27(35):9503-12
15. McClatchy DB, Liao L, Park SK, Venable J, and Yates JR. Quantification of the synaptic proteome of the rat cerebellum during postnatal development. Genome Res. 2007; 17(9):1378-88
16. Liao L, Pilotte J, Xu T, Wong CL, Edelman GM, Vanderklish PW, and Yates JR. BDNF induces widespread changes in synaptic protein content and upregulates the translation machinery components: an analysis using high-throughput proteomics. J Proteome Res. 2007; 6(3):1059-71
17. Liao L, Cheng D, Wang J, Losik T, Duong D, Gearing M, Rees H, Lah JJ, Levey AI, Peng J. Proteomic characterization of postmortem amyloid plaques isolated by laser capture microdissection. J Biol Chem. 2004; 279(35): 37061-8